Introduction: Mesenchymal stem cells (MSCs) are multipotent cells able to differentiate into several mesenchymal\r\nlineages, classically derived from bone marrow (BM) but potentially from umbilical cord blood (UCB). Although they\r\nare becoming a good tool for regenerative medicine, they usually need to be expanded in fetal bovine serum\r\n(FBS)-supplemented media. Human platelet lysate (HPL) has recently been proposed as substitute for safety\r\nreasons, but it is not yet clear how this supplement influences the properties of expanded MSCs.\r\nMethods: In the present study, we compared the effect of various media combining autologous HPL with or\r\nwithout FBS on phenotypic, proliferative and functional (differentiation, cytokine secretion profile) characteristics of\r\nhuman BM-derived MSCs.\r\nResults: Despite less expression of adipogenic and osteogenic markers, MSCs cultured in HPL-supplemented media\r\nfully differentiated along osteoblastic, adipogenic, chondrogenic and vascular smooth muscle lineages. The analyses\r\nof particular specific proteins expressed during osteogenic differentiation (calcium-sensing receptor (CaSR) and\r\nparathormone receptor (PTHR)) showed their decrease at D0 before any induction for MSC cultured with HPL\r\nmostly at high percentage (10%HPL). The cytokine dosage showed a clear increase of proliferation capacity and\r\ninterleukin (IL)-6 and IL-8 secretion.\r\nConclusions: This study shows that MSCs can be expanded in media supplemented with HPL that can totally\r\nreplace FBS. HPL-supplemented media not only preserves their phenotype as well as their differentiation capacity,\r\nbut also shortens culture time by increasing their growth rate.
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